abstract：:Understanding the genetics of biological diversification across micro- and macro-evolutionary time scales is a vibrant field of research for molecular ecologists as rapid advances in sequencing technologies promise to overcome former limitations. In palms, an emblematic, economically and ecologically important plant f...

journal_title：Molecular ecology resources

authors： de La Harpe M,Hess J,Loiseau O,Salamin N,Lexer C,Paris M

更新日期：2019-01-01 00:00:00

abstract：:In this article, we describe ednaoccupancy, an r package for fitting Bayesian, multiscale occupancy models. These models are appropriate for occupancy surveys that include three nested levels of sampling: primary sample units within a study area, secondary sample units collected from each primary unit and replicates o...

journal_title：Molecular ecology resources

authors： Dorazio RM,Erickson RA

更新日期：2018-03-01 00:00:00

abstract：:Molecular markers have been used to identify the sex of sampled individuals for several decades, but the time-consuming development phase prevented their application in many systems. Recently, a growing number of papers have applied reduced-representation sequencing (RRS) protocols to the identification of sex-specifi...

journal_title：Molecular ecology resources

authors： Moore JS,Benestan L

更新日期：2018-03-01 00:00:00

abstract：:Next-generation sequencing is a common method for analysing microbial community diversity and composition. Configuring an appropriate sequence processing strategy within the variety of tools and methods is a nontrivial task and can considerably influence the resulting community characteristics. We analysed the V4 regi...

journal_title：Molecular ecology resources

authors： Hardge K,Neuhaus S,Kilias ES,Wolf C,Metfies K,Frickenhaus S

更新日期：2018-03-01 00:00:00

abstract：:Local adaptation is often studied via (i) multiple common garden experiments comparing performance of genotypes in different environments and (ii) sequencing genotypes from multiple locations and characterizing geographic patterns in allele frequency. Both approaches aim to characterize the same pattern (local adaptat...

journal_title：Molecular ecology resources

authors： Lasky JR,Forester BR,Reimherr M

更新日期：2018-01-01 00:00:00

abstract：:Female Drosophila melanogaster frequently mate with multiple males in nature as shown through parentage analysis. Although polyandry is well documented, we know little about the timing between mating events in wild Drosophila populations due to the challenge of following behaviours of individual females. In this study...

journal_title：Molecular ecology resources

authors： Giardina TJ,Clark AG,Fiumera AC

更新日期：2017-11-01 00:00:00

abstract：:The spatial signature of microevolutionary processes structuring genetic variation may play an important role in the detection of loci under selection. However, the spatial location of samples has not yet been used to quantify this. Here, we present a new two-step method of spatial outlier detection at the individual ...

journal_title：Molecular ecology resources

authors： Wagner HH,Chávez-Pesqueira M,Forester BR

更新日期：2017-11-01 00:00:00

abstract：:Data on hundreds or thousands of single nucleotide polymorphisms (SNPs) provide detailed information about the relationships between individuals, but currently few tools can turn this information into a multigenerational pedigree. I present the r package sequoia, which assigns parents, clusters half-siblings sharing a...

journal_title：Molecular ecology resources

authors： Huisman J

更新日期：2017-09-01 00:00:00

abstract：:Plastid sequencing is an essential tool in the study of plant evolution. This high-copy organelle is one of the most technically accessible regions of the genome, and its sequence conservation makes it a valuable region for comparative genome evolution, phylogenetic analysis and population studies. Here, we discuss re...

journal_title：Molecular ecology resources

authors： Twyford AD,Ness RW

更新日期：2017-09-01 00:00:00

abstract：:The 'Symphyta' is a paraphyletic assemblage at the base of the order Hymenoptera, comprising 14 families and about 8750 species. All have phytophagous larvae, except for the Orussidae, which are parasitoids. This study presents and evaluates the results of DNA barcoding of approximately 5360 specimens of 'Symphyta', m...

journal_title：Molecular ecology resources

authors： Schmidt S,Taeger A,Morinière J,Liston A,Blank SM,Kramp K,Kraus M,Schmidt O,Heibo E,Prous M,Nyman T,Malm T,Stahlhut J

更新日期：2017-07-01 00:00:00

abstract：:The formation of chimeric sequences can create significant methodological bias in PCR-based DNA metabarcoding analyses. During mixed-template amplification of barcoding regions, chimera formation is frequent and well documented. However, profiling of fungal communities typically uses the more variable rDNA region ITS....

journal_title：Molecular ecology resources

authors： Bjørnsgaard Aas A,Davey ML,Kauserud H

更新日期：2017-07-01 00:00:00

abstract：:Microsatellite markers have played a major role in ecological, evolutionary and conservation research during the past 20 years. However, technical constrains related to the use of capillary electrophoresis and a recent technological revolution that has impacted other marker types have brought to question the continued...

journal_title：Molecular ecology resources

authors： De Barba M,Miquel C,Lobréaux S,Quenette PY,Swenson JE,Taberlet P

更新日期：2017-05-01 00:00:00

abstract：:Archived specimens are highly valuable sources of DNA for retrospective genetic/genomic analysis. However, often limited effort has been made to evaluate and optimize extraction methods, which may be crucial for downstream applications. Here, we assessed and optimized the usefulness of abundant archived skeletal mater...

journal_title：Molecular ecology resources

authors： Nielsen EE,Morgan JAT,Maher SL,Edson J,Gauthier M,Pepperell J,Holmes BJ,Bennett MB,Ovenden JR

更新日期：2017-05-01 00:00:00

abstract：:The pygmy grasshopper Tetrix japonica is a common insect distributed throughout the world, and it has the potential for use in studies of body colour polymorphism, genomics and the biology of Tetrigoidea (Insecta: Orthoptera). However, limited biological information is available for this insect. Here, we conducted a d...

journal_title：Molecular ecology resources

authors： Qiu Z,Liu F,Lu H,Huang Y

更新日期：2017-05-01 00:00:00

abstract：:Microbial fitness is easy to measure in the laboratory, but difficult to measure in the field. Laboratory fitness assays make use of controlled conditions and genetically modified organisms, neither of which are available in the field. Among other applications, fitness assays can help researchers detect adaptation to ...

journal_title：Molecular ecology resources

authors： Boynton PJ,Stelkens R,Kowallik V,Greig D

更新日期：2017-05-01 00:00:00

abstract：:This article documents the whole genome sequence information of the Indian Zaprionus indianus, a member of the fruit fly family Drosophilidae. The sequences were generated on an Illumina platform and reads and whole genome sequence submitted to NCBI to the SRA and BioProject databases, respectively. This is the first ...

journal_title：Molecular ecology resources

authors： Khanna R,Mohanty S

更新日期：2017-05-01 00:00:00

abstract：:Single nucleotide polymorphism (SNP) markers were identified and validated for two stingrays species, Potamotrygon motoro and Potamotrygon falkneri, using double digest restriction-site associated DNA (ddRAD) reads using 454-Roche technology. A total of 226 774 reads (65.5 Mb) were obtained (mean read length 289 ± 183...

journal_title：Molecular ecology resources

authors： Cruz VP,Vera M,Pardo BG,Taggart J,Martinez P,Oliveira C,Foresti F

更新日期：2017-05-01 00:00:00

abstract：:Two recent articles were written in response to our paper "Breaking RAD: An evaluation of the utility of restriction site associated DNA sequencing scans of adaptation." While we agree with some of the comments made by the authors of these two response papers, we still believe caution should be employed in RADseq stud...

journal_title：Molecular ecology resources

authors： Lowry DB,Hoban S,Kelley JL,Lotterhos KE,Reed LK,Antolin MF,Storfer A

更新日期：2017-05-01 00:00:00

abstract：:We introduce phylodyn, an r package for phylodynamic analysis based on gene genealogies. The package's main functionality is Bayesian nonparametric estimation of effective population size fluctuations over time. Our implementation includes several Markov chain Monte Carlo-based methods and an integrated nested Laplace...

journal_title：Molecular ecology resources

authors： Karcher MD,Palacios JA,Lan S,Minin VN

更新日期：2017-01-01 00:00:00

abstract：:High-throughput sequencing has changed many aspects of population genetics, molecular ecology and related fields, affecting both experimental design and data analysis. The software package angsd allows users to perform a number of population genetic analyses on high-throughput sequencing data. angsd uses probabilistic...

journal_title：Molecular ecology resources

authors： Durvasula A,Hoffman PJ,Kent TV,Liu C,Kono TJ,Morrell PL,Ross-Ibarra J

更新日期：2016-11-01 00:00:00

abstract：:New DNA sequencing technologies are allowing researchers to explore the genomes of the millions of natural history specimens collected prior to the molecular era. Yet, we know little about how well specific next-generation sequencing (NGS) techniques work with the degraded DNA typically extracted from museum specimens...

journal_title：Molecular ecology resources

authors： McCormack JE,Tsai WL,Faircloth BC

更新日期：2016-09-01 00:00:00

abstract：:Restriction-site-associated DNA sequencing (RAD-seq) and related methods are revolutionizing the field of population genomics in nonmodel organisms as they allow generating an unprecedented number of single nucleotide polymorphisms (SNPs) even when no genomic information is available. Yet, RAD-seq data analyses rely o...

journal_title：Molecular ecology resources

authors： Rodríguez-Ezpeleta N,Bradbury IR,Mendibil I,Álvarez P,Cotano U,Irigoien X

更新日期：2016-07-01 00:00:00

abstract：:We present the leaf and floral transcriptomes of two hybridizing bromeliad species that differ in their major pollinator systems. Here we identified candidate genes responsible for pollinator attraction and reproductive isolation in these two species. We searched for candidate genes involved in floral traits, such as ...

journal_title：Molecular ecology resources

authors： Palma-Silva C,Ferro M,Bacci M,Turchetto-Zolet AC

更新日期：2016-07-01 00:00:00

abstract：:Recent advances in high-throughput sequencing technologies have offered the possibility to generate genomewide sequence data to delineate previously unidentified genetic structure, obtain more accurate estimates of demographic parameters and to evaluate potential adaptive divergence. Here, we identified 27 556 single ...

journal_title：Molecular ecology resources

authors： Zhang BD,Xue DX,Wang J,Li YL,Liu BJ,Liu JX

更新日期：2016-05-01 00:00:00

abstract：:The sea urchin Arbacia lixula is a keystone species in Mediterranean ecosystems that drive landscape changes in littoral communities. However, genomic information available for the whole order Arbacioida is very limited. Using RNA-seq techniques, we have characterized the transcriptome of four different tissue types i...

journal_title：Molecular ecology resources

authors： Pérez-Portela R,Turon X,Riesgo A

更新日期：2016-05-01 00:00:00

abstract：:Biodiversity analyses based on next-generation sequencing (NGS) platforms have developed by leaps and bounds in recent years. A PCR-free strategy, which can alleviate taxonomic bias, was considered as a promising approach to delivering reliable species compositions of targeted environments. The major impediment of suc...

journal_title：Molecular ecology resources

authors： Liu S,Wang X,Xie L,Tan M,Li Z,Su X,Zhang H,Misof B,Kjer KM,Tang M,Niehuis O,Jiang H,Zhou X

更新日期：2016-03-01 00:00:00

abstract：:Type specimens have high scientific importance because they provide the only certain connection between the application of a Linnean name and a physical specimen. Many other individuals may have been identified as a particular species, but their linkage to the taxon concept is inferential. Because type specimens are o...

journal_title：Molecular ecology resources

authors： Prosser SW,deWaard JR,Miller SE,Hebert PD

更新日期：2016-03-01 00:00:00

abstract：:A variety of challenges arise when monitoring wildlife populations for disease. Sampling tissues can be invasive to hosts, and obtaining sufficient sample sizes can be expensive and time-consuming, particularly for rare species and when pathogen prevalence is low. Environmental DNA (eDNA)-based detection of pathogens ...

journal_title：Molecular ecology resources

authors： Hall EM,Crespi EJ,Goldberg CS,Brunner JL

更新日期：2016-03-01 00:00:00

abstract：:Sequence-based specimen identification, known as DNA barcoding, is a common method complementing traditional morphology-based taxonomic assignments. The fundamental resource in DNA barcoding is the availability of a taxonomically reliable sequence database to use as a reference for sequence comparisons. Here, we provi...

journal_title：Molecular ecology resources

authors： Barco A,Raupach MJ,Laakmann S,Neumann H,Knebelsberger T

更新日期：2016-01-01 00:00:00

abstract：:Photosynthetic eukaryotes have a critical role as the main producers in most ecosystems of the biosphere. The ongoing environmental metabarcoding revolution opens the perspective for holistic ecosystems biological studies of these organisms, in particular the unicellular microalgae that often lack distinctive morpholo...

journal_title：Molecular ecology resources

authors： Decelle J,Romac S,Stern RF,Bendif el M,Zingone A,Audic S,Guiry MD,Guillou L,Tessier D,Le Gall F,Gourvil P,Dos Santos AL,Probert I,Vaulot D,de Vargas C,Christen R

更新日期：2015-11-01 00:00:00

abstract：:Degraded DNA from suboptimal field sampling is common in molecular ecology. However, its impact on techniques that use restriction site associated next-generation DNA sequencing (RADSeq, GBS) is unknown. We experimentally examined the effects of in situDNA degradation on data generation for a modified double-digest RA...

journal_title：Molecular ecology resources

authors： Graham CF,Glenn TC,McArthur AG,Boreham DR,Kieran T,Lance S,Manzon RG,Martino JA,Pierson T,Rogers SM,Wilson JY,Somers CM

更新日期：2015-11-01 00:00:00

abstract：:This article documents the public availability of (i) microbiomes in diet and gut of larvae from the dipteran Dilophus febrilis using massive parallel sequencing, (ii) SNP and SSR discovery and characterization in the transcriptome of the Atlantic mackerel (Scomber scombrus, L) and (iii) assembled transcriptome for an...

journal_title：Molecular ecology resources

authors： Genomic Resources Development Consortium.,Álvarez P,Arthofer W,Coelho MM,Conklin D,Estonba A,Grosso AR,Helyar SJ,Langa J,Machado MP,Montes I,Pinho J,Rief A,Schartl M,Schlick-Steiner BC,Seeber J,Steiner FM,Vilas C

更新日期：2015-11-01 00:00:00

abstract：:Most hosts are concurrently or sequentially infected with multiple parasites; thus, fully understanding interactions between individual parasite species and their hosts depends on accurate characterization of the parasite community. For parasitic nematodes, noninvasive methods for obtaining quantitative, species-speci...

journal_title：Molecular ecology resources

authors： Budischak SA,Hoberg EP,Abrams A,Jolles AE,Ezenwa VO

更新日期：2015-09-01 00:00:00

abstract：:Linkage disequilibrium (LD, association of allelic states across loci) is poorly understood by many evolutionary biologists, but as technology for multilocus sampling improves, we ignore LD at our peril. If we sample variation at 10 loci in an organism with 20 chromosomes, we can reasonably treat them as 10 'independe...

journal_title：Molecular ecology resources

authors： Baird SJ

更新日期：2015-09-01 00:00:00

abstract：:Quantifying and classifying ichthyoplankton is one of the most effective ways of monitoring the recruitment process in fishes. However, correctly identifying the fish based on morphological characters is extremely difficult, especially in the early stages of development. We examined ichthyoplankton from tributaries an...

journal_title：Molecular ecology resources

authors： Frantine-Silva W,Sofia SH,Orsi ML,Almeida FS

更新日期：2015-09-01 00:00:00

abstract：:Germplasm collections provide an extremely valuable resource for breeders and researchers. However, misclassification of accessions by species often hinders the effective use of these collections. We propose that use of high-throughput genotyping tools can provide a fast, efficient and cost-effective way of confirming...

journal_title：Molecular ecology resources

authors： Mason AS,Zhang J,Tollenaere R,Vasquez Teuber P,Dalton-Morgan J,Hu L,Yan G,Edwards D,Redden R,Batley J

更新日期：2015-09-01 00:00:00

abstract：:Knowledge of population demographics is important for species management but can be challenging in low-density, wide-ranging species. Population monitoring of the endangered Sonoran pronghorn (Antilocapra americana sonoriensis) is critical for assessing the success of recovery efforts, and noninvasive DNA sampling (ND...

journal_title：Molecular ecology resources

authors： Woodruff SP,Johnson TR,Waits LP

更新日期：2015-07-01 00:00:00

abstract：:Evolutionary alterations to cis-regulatory sequences are likely to cause adaptive phenotypic complexity, through orchestrating changes in cellular proliferation, identity and communication. For nonmodel organisms with adaptive key innovations, patterns of regulatory evolution have been predominantly limited to targete...

journal_title：Molecular ecology resources

authors： Kratochwil CF,Meyer A

更新日期：2015-07-01 00:00:00

abstract：:Single nucleotide polymorphisms (SNPs) have become the marker of choice for genetic studies in organisms of conservation, commercial or biological interest. Most SNP discovery projects in nonmodel organisms apply a strategy for identifying putative SNPs based on filtering rules that account for random sequencing error...

journal_title：Molecular ecology resources

authors： Gonçalves da Silva A,Barendse W,Kijas JW,Barris WC,McWilliam S,Bunch RJ,McCullough R,Harrison B,Hoelzel AR,England PR

更新日期：2015-07-01 00:00:00

abstract：:We combined restriction site associated DNA sequencing (RADseq) using a hypomethylation-sensitive enzyme and messenger RNA sequencing (mRNAseq) to develop molecular markers for the 16 gigabase genome of Cedrus atlantica, a conifer tree species. With each method, Illumina(®) reads from one individual were used to gener...

journal_title：Molecular ecology resources

authors： Karam MJ,Lefèvre F,Dagher-Kharrat MB,Pinosio S,Vendramin GG

更新日期：2015-05-01 00:00:00